Journal: Scientific Reports
Article Title: Long-term electrical stimulation at ear and electro-acupuncture at ST36-ST37 attenuated COX-2 in the CA1 of hippocampus in kainic acid-induced epileptic seizure rats
doi: 10.1038/s41598-017-00601-1
Figure Lengend Snippet: Western blot findings for the effects of electrical stimulation at the ear and electro-acupuncture at ST36–ST37 on the levels of COX-2, GFAP, S-100B, RAGE, mGluR3, MCP-1, and CCR2 in kainic acid (KA)-induced epileptic seizure rats. The levels of COX-2 increased in the KA group (K) and sham group (S), and decreased in the auricular group (E) and Zusanli group (Z) ( A and B ). The levels of GFAP increased in the KA group and sham group and decreased in the auricular group and Zusanli group ( A and C ). The levels of S100-B ( A and D ), RAGE ( A and E ), mGluR3 ( A and F ), MCP-1 ( A and G ), and CCR2 ( A and H ) among the normal group (N) and the KA, sham, auricular, and Zusanli groups. ¶ p < 0.05 compared with the value of N; *p < 0.05 compared with the value of K; # p < 0.05 compared with the value of S; n = 6.
Article Snippet: The brain sections were blocked for 10 min in PBS containing 10% bovine serum albumin (Sigma, USA) and then incubated overnight at 4 °C with the primary antibodies [1:1000 rabbit polyclonal S-100B (Novus bio, USA) and 1:500 mouse monoclonal GFAP (Calbiochem, USA)].
Techniques: Western Blot